実験的慢性胃潰瘍の組織化学的研究

第II編 各種薬剤による影響

抄録

In this paper, the results of the studies on histochemical changes in experimental ulcers in cases of prolongation and accelerated healing were reported. In this study, the Clamping-Cortisone ulcer in rats were used. This experimental ulcer was made by the procedures mentioned before. In the histochemical studies, PAS, Alcian-blue, TBM, Aldehyde-fuchsin and Neutral red staining methods were used for examing polysaccharides in the tissues. Further, Gormori's and Azo staining methods were used for staining alkaline-phosphatase. The animals with C-C ulcer were divided into three groups, i.e. the C-C ulcer group treated continuously with cortisone during the experimental period, (named C-C-C group), the group treated with an anabolic steroid, 4Cl-TA (the C-C-A group) and the group treated with an anti ulcerous drug, basic aluminum sucrose sulfate (ulcermin), (the C-C-U group), The PAS reaction of the mucous content in the regenerative mucosa and connective tissue at the base of the ulcer developed remarkably in the early stage of healing in both the C-C-A and C-C-U groups, but was poor in the C-C-C group. The PAS reaction of the connective tissue was also remarkable in the two former groups, but was poor in the latter group. The Alcian-blue staining activities were the same as those of PAS, but fell in the latter stages of the healing of the ulcers. In the C-C-C group, the regenerative mucosal cells, the connective tissue, infiltrative neutrophile and capillary wall showed only a slight positive activity of alkaline phosphatase. In the groups treated with basic aluminum sucrose sulfate and 4Cl-TA, the regenerative mucosa and capillary wall in the connective tissue showed remarkable activity compared with that of the control group.
From these findings, it appeared that the reasons why healing of the C-C-C ulcer was prolonged are an inhibition of mucous secreation from regenerative mucous cells and also poor development of polysaccharides and alkaline phosphatase in the connective tissues. On the centrary, the curative effects of 4Cl-TA and basic aluminum sucrose sulfate may be attributed to an accelerating activity of these agents on secretion of the mucous and on the formation of polysaccharides and alkaline phosphatase in the connective tissues.