Bilirubin glucuronide 分画抽出法 (Talafant 法) の検討

抄録

We studied Talafant's method for the preparation of bilirubin diglucuronide and obtained the results as follows.
1) The molar ratio of bilirubin glucuronide fraction prepared by this method proves to be 1.98 in average, which is quite close to the theoretical value in its purity.
2) Spectrophotometric properties of this fraction coincide well with those of the estertype bilirubin that we know in our laboratory.
3) On the paper chromatography this fraction separates into two spots one of which proves to be a contaminant.
4) The amount of the bilirubin glucuronide fraction harvested by our method is quite minimal (being 2.78% in average), but it is somewhat better than by the original method of Talafant. This seems to be due to the fact that we used the so-called 'crude bilirubin' of Sakamoto et al. as the material.