A Kinetic Study of ¹³¹I-BSP in Rat with Sequential Micro-Sampling Method

Abstract

Relative large amount of blood must be drawn to observe kinetics of sulfobromophthalein in liver according to the conventional colorimetric procedures.
It might be inadequate to get correct informations about the dye metabolism in a small experimental animal because of excessive blood loss in these procedures.
Therefore, new micro-sampling method for dynamic study of ¹³¹I-BSP in a rat is divised and this does not show any serious affects on its physiological conditions.
After injecting 0.025mg/0.5ml of ¹³¹I-BSP into rat tail vein, more than 10 blood samples of 0.07ml each are able to be sequentially collected with microhematocrit tubes.
Mean T1/2, blood disappearance rate and blood retention rate in 10 normal control rats are 1.16min., 0.606, 0.69% in 40min., on the contrary, these values in 7 CC₄-administered rats are 1.90min., 0.383, 1.46% respectively.
This new method seemed to be quite valuable for study of excretion mechanismus of ¹³¹I-BSP in a small experimental animal.