Experimental gastric xanthomatosis on rabbits

Abstract

Gastric xanthomatosis are frequently found in men but their production in the experimentalanimals has not been reported so far. Recently we found gastric xanthomatous polyp and gastric xanthomatosis in rabbits who had been treated with chlormadinon acetate undergoing cholesterol feeding. Twenty male albino rabbits aged 6 to 7months were divided into two groups; ten control group of rabbits were given orally one capsul of potato starch as placebo daily for 15weeks (placebo control group), another ten was given 1mg/kg/day of chlormadinon acetate for 15weeks (chlormadinon acetat group). Both groups were fed by 1% cholesterol for the same period as well. During the course of the experiments the body weight were record weekly and serum cholesterol level were mesured every two weeks. After 15weeks all rabbits were sacrificed for pathological examination. One rabbits died during the course of the experiment in placebo control group and chlormadinon acetate group were all survived for 15weeks. After 15weeeks serum cholesterol level were 1599.2±251.8 (SE) mg/dl on placebo control group and 1690.8±176.8 (SE) mg/dl on chlormadinon acetate group. The most characteristic findings of the stomach were polypoid and granular lesion on cardia and its neighbor with rabbits of chlormadinon acetate group. On histological examination thesepolypoid and granular lesion were occupied by foamy cells and these foamy cells were stained to be bright red by Sudan 111. There are many sudanofilic cells on the propria mucosa of other part of gastric mucosa. These histological findings are similar to gastric xanthoma found in human stomach. Placebo control group did not showed any polypoid and granular lesion by macroscopic examination, but on the histological examination some sudanofilic cells were scarecely found on the portion of propria mucosa. These findings sugest that the conbination of chlormadinon acetate and hyperlipemia may play an important role for the genesis of experimental gastric xanthomatosis and may be used for the study on etiology of human gastric xantoma.