1977 Volume 74 Issue 5 Pages 581-588
It is considered that the development of new animal models is essential for the study of early diagnosis and curable treatment of the pancreatic carcinoma. The following experiments were carried out in order to investigate the carcinogenic effect of Diisopropanolnitrosamine (DIPN). DIPN in olive oil was administered subcutaneously once a week to male golden hamsters at 3 dose levels (250, 500 and 1000 mg/kg body weight) and the animals were sacrificed after twenty weeks. In 250 mg group, the hyperplasia of pancreatic ducts of minimal degree was seen, but no neoplastic change was observed. In 500 mg group, tumor nodules were grossly recognized in some cases and papillary hyperplasia of pancreatic duct-cell, ductal adenomas or ductal adenocarcinomas were observed in all cases microscopically. The ductal adenocarcinomas coexisted with adenomas were found in all but one of eight cases. These neoplasms resembled human pancreatic tumors. Outside the pancreas, cholangiocarcinoma and adenoma of the lung were seen in 25% of the cases respectively. The findings in 1000 mg group were similar to 500 mg group. These results suggest that the subcutaneous administration of DIPN is one of the best methods to induce the pancreatic carcinoma experimentally.