Revaluation of the methods for chronic pancreatic fistula in dog

Developement of our improved method

Abstract

From our study revaluating various types of chronic pancreatic fistula reported in the past, it is concluded that the method for construction of a duodenal pouch draining pancreatic juiced workes for a long period under a stable condition and provides physiological response in spite of partial denervation of the pancreas and contamination of the duodenal fluid in the pancreatic juice. It was found that major points for construction of chronic pancreatic fistula werc a placement of draining apparatus in the pouch and the duodenum and instillation of pancreatic juice into the duodenum. Based upon these observation, we developed our own method. In this procedure, pancreatic juice secreted into the duodenal pouch was induced to the outside of the body with a negative pressure at 25cmH₂O. The juice was detected by means of photo-switch and returned into the body through the duodenal fistula with a peristaltic pump. Since our method enables us to record continuously secretory changes of the pancreatic juice for a long period of time under physiological condition and ti was found that secretory response to secretin or CCK was similar to that reported by others, our improved method will be very useful for studies in exocrine pancreas.