SERIAL DETERMINATION OF SERUM GOT ISOZYME ACTIVITIES FOR THE EVALUATION OF ACUTE HEPATIC DAMAGE

Abstract

Serum glutamic oxaloacetic transaminase (GOT) isozyme activities were serially measured by an immunological method in 4 cases of hepatic damage following a transient circulatory failure or anoxia episode. When the GOT isozyme activities were plotted on semilogarithmic paper, the activities decreased in a straight line during the first three days after an circulatory disturbance episode. The disappearance rates of GOT isozyme activities were almost same in all cases. The apparent half-life of mitochondrial GOT (GOT-m) was calculated as 0.46 days, while that of cytozolic GOT (GOT-s) was 0.63 days. The disappearance rates of GOT isozymes were analyzed in three acute hepatic disorders with good prognosis, two cases of halothane hepatitis and a case of fulminant hepatitis. When GOT-m activity decreased half in about 0.46 days, the pathogenic factors which were attributed to necrosis of hepatic parenchymal cells were thought disappeared. It was concluded that analysis of GOT isozyme disappearance rates gave usefull informations to evaluate acute hepatic damage.