INFLUENCE OF SYNTHETIC ATP ON DYE EXCRETING FUNCTION OF THE LIVER IN ATP DEFICIENT RAT

Abstract

Biliary excretion of BSP or ICG and the influence of synthetic ATP thereupon were examined on the ATP deficient rat through an intraperitoneal injection of d-frunctose (20mmole/Kg b.w.). the following results were obtained.
1) Content of ATP in the whole liver homogenate was increased through either intraperitoneal or oral administration of synthetic ATP, although it was less and slow through the latter.
2) The hepatic content of ATP was decreased through an intraperitoneal injection of dfructose.
3) An intraperitoneal administration of d-fructose decreased the hepatic content of Y and Z proteins, but the activity of hepatic microsomal cytochrome P-450 and the hepatic uptake of ³H-bilirubin were unchanged. On electron microscopy no distinct abnormality was noticed in the hepatocyte and bile ductule.
4) The decrease of hepatic content of ATP through an intraperitoneal injection of d-fructose was inhibited by either an oral or intraperitoneal administration of synthetic ATP.
5) Bile output and biliary excretion of BSP or ICG were decreased through an intraperitoneal injection of d-fructose. In general, the decrease of biliary excretion of dyes was based on thediminished bile output.
6) The decrease of bile output and biliary excretion of BSP through d-fructose were inhibited by an administration of synthetic ATP, but the inhibition was not clear in the case of ICG excretion.
It was concluded that hepatic ATP is indispensable to the mechanism of bile excretion and that either oral or intraperitoneal administration of synthetic ATP can increase the hepatic content of ATP and participate in the mechanism of biliary excretion of dyes.