PLASMA SECRETIN LEVEL AND EXOCRINE PANCREATIC SECRETION IN RESPONSE TO INTRADUODENAL INFUSION OF l-PHENYL-l-HYDROXY-N-PENTANE AND HC1 BY USING NEW FOUR LUMEN TUBE

Abstract

We have designed four lumen tube which have enabled us to aspirate duodenal juice infusing endogenous stimuli into the lower part of the duodenum. Using this tube, exocrine pancreatic secretion and immunoreactive secretin (IRS) level have been simultaneously observed in response to intraduodenal infusion of l-phenyl-l-hydroxy-n-pentane (PHP) and 0.1N HCl in normal subjects, patients with diabetes mellitus, and patients with chronic pancreatitis.
1) Pancreatic flow rate and bicarbonate concentration have increased as two times as those of basal level after infusion of PHP or HCl in normal subjects. IRS levels have also increased correspondently in normal subjects. The same tendency have been observed in patients with diabetes mellitus.
2) Exocrine pancreatic secretion have not changed after infusion of PHP or HCl in patients with chronic pancreatitis. Pancreatic flow rate, bicarbonate concentration and amylase output have been significant low in patients with chronic pancreatitis compared with those of normal subjects and patients with diabetes mellitus.
3) Pancreatic flow rate and bicarbonate concentration have increased as about two times as those of basal level after both PHP and HCl infusion. On the other hand, although pancreatic amylase output has increased significantly after infusion of HC1 compared with basal secretion, it has not increased after infusion of PHP. Therefore we have concluded that PHP has been more specific substance for releasing secretin than HCl.