THE IMMUNOFLUORESCENCE STUDY OF GASTRIN PRODUCING CELL IN THE INTERMEDIATE ZONE OF GASTRIC MUCOSA

I. Its relationship to the Atrophic Border of Atrophic Gastritis

Abstract

Localization of gastrin (G) cell in the intermediate zone was observed by indirect im-munofluorescence method. The samples of gastric mucosa, obtained by endoscopic biopsy or from resected stomach, were fixed with pre-cooled 95% ethanol, and stained with rabbit anti-gastrin serum (CIS) and FITC-labelled goat anti rabbit IgG serum.
At first, we confirmed the existance of G cells in the intermediate zone. The G cell population was dominant in the mid-zone and rare in the lower-third of the glands in the intermediate zone. Futhermore, the G cells were seen at the fundic side of the atrophic border, in where fundic glands were replaced with pseudopyloric glands.
These results presented herin strongly suggested that the G cells might newly appear at the intermediate zone with the spread of atrophic gastritis.