STUDY ON DISACCHARIDASE ACTIVITY OF BIOPSY SPECIMENS

Part II: Especially on Intestinal Metaplasia

Abstract

We measured disaccharidase activity (maltase, sucrase and lactase) of intestinal metaplasia by endoscopic dyeing method with gascromatography and had enzymological study on it.
We measured those activity of gastric cancer (focus) and intestinal metaplasia in surrounding focus and 1.5cm-2.0cm from focus.
1) Comparing disaccharidase activity of stained region and unstained region, that of stained region was higher. It was that there were many factors in endoscopic dyeing method to recognize disaccharidase activity in also unstained region. So we need further study about this fact.
2) Disaccharidase activity in each of the stain types showed almost same value.
3) As stainning degree was stronger, activity was higher.
4) Disaccharidase activity was not recognized in fundic area. Concerning to maltase and sucrase, there was no difference between the two value in intestinal metaplasia and duodenal bulb. Lactase showed 0.07/g min. in intestinal metaplasia and 0.12/g min. in duodenal bulb.
5) Maltase and lactase activity had no difference in each parts of early gastric cancer and advanced gastric cancer. Maltase and lactase activity were higher in each parts of well differentiated type than that of poorly differentiated type.
6) As the distance from focus was longer, maltase and lactase were higher, and those of intestinal metaplasia in intact stomach without gastric cancer were highest.