1980 Volume 77 Issue 12 Pages 1915-1922
The cholestatic factors were produced from the sensitized lymphocytes by specific antigenic stimulation in vitro. These active principles were fractionated by a gel filtration using Sephadex G-75 column followed by DEAE-cellulose column chromatography. Two active fractions obtained by a latter chromatography exhibited a marked intrahepatic cholestasis when they were infused into the mesenteric vein of rats. Although the cholestatic activity of these two fractions did not influence by RNase or DNase digestion, their activities were completely inactivated by treatment of either trypsin or neuraminidase.
These results suggest that the cholestatic factors may have a glycoprotein nature.