FATE AND ROLE OF SHG IN THE CANINE GASTRIC POUCH

Abstract

Fate and role of the gastrin in the gastric lumen was studied. (1) Twenty dogs were subjected to complete removal of G-I tract except the fundic area of the stomach which were utilized as a gastric pouch. Synthetic human gastrin (SHG) or ¹²⁵I-SHG were infused into the fundic pouch and blood samples from portal vein were collected for determining gastrin concentration. (2) Fundic pouch together with feeding artery and draining vein were isolated from 5 dogs and perfused with oxygenated blood in a small chamber containing lactate-Ringer's solution. SHG was infused into the fundic pouch and blood samples were collected from draining vein. Using newly deviced intra-gastric titration system, all these fundic pouches were perfused to maintain the intra-gastric pH to 8.4.
Results, (1) SHG in the gastric perfusates (pH 8.4) is transferred into the blood circulation, (2) luminal gastrin (SHG) hardly participates in the stimulation of the acid secretion.