1981 Volume 78 Issue 10 Pages 1920-1928
Institute of Gastroenterology and Internal Medicine, Tokyo Women's Medical School A sensitive, specific and reproducible radioimmunoassay for secretin has been developed. Synthetic procine secretin was iodinated by a modification of chloramine-T method. Purification and separation of labelled from unlabelled secretin was carried out by a two-step procedure incorporating gel filtration and ion-excharge chromatography. The labelled preparation had a specific radioactivity of 150-200μCi/μg.
Plasma secretin was purified and concentrated by ethanol extraction for eliminating a nonspecific interfering substance (s) in plasma with assay system. The detection limit was 10pg/ml. Intra-and inter-assay precision was 51.±3.1 and 16.8±1.6pg/ml (mean±S. D.), respectively. Plasma secretin concentrations in response to ingestion of a test meal were studied in 15 healthy subjects. The mean fasting plasma secretin concentration was 21.1±1.4pg/ml (mean±S. E.). Ingestion of a meal resulted in significant increase in the plasma secretin concentrations. This result apparently indicates that secretin plays a physiological role in stimulating the pancreatic secretion after a meal in man.
Nippon Shokakibyo Gakkai Zasshi
Nippon Shokakibyo Gakkai Zasshi Kou
