STUDIES ON RIBONUCLEASES IN THE PANCREATIC JUICE OF PATIENTS WITH PANCREATIC DISEASE

I. Ribonuclease Assay, Effect of Storing Samles and Isolation of the Ribonucleases in Pure Pancreatic juice

Abstract

The assay method of ribonuclease (RNase) activity in duodenal juice after ancreozyminsecretin test and the effect of storing samles were studied. Furthermore, the RNase in ure ancreatic juice was searated on hoshocellulose column chromatograhy.
The otimal H of the incubation medium for determination of RNase activity in the duodenal juice, when poly C or poly U was used as the substrate, was 6.0 and 5.5 resectively. RNase activity in the duodenal juice was comletely lost when ket at 80°C for more than half an hour or at room temerature for more than a day. The enzyme was reduced to 78% after 3 months, even if it was ket at -20°C. Whereas, 100% of the activity was reserved at least for 3 months, when arotinin was added to the samle and it was ket at -20°C. Therefore the samle should be ket at -70°C and assayed in a week.
By searation of roteins in the ure ancreatic juice on hoshocellulose chromatograhy, three eaks of RNase activity were observed and all of these RNases exhibited a higher reference to poly C than poly U. Poly A or oly G were not hydrolysed by these fractions. By this method, RNase in the ancreatic juice was searated from immunoreactive trysin.