1982 Volume 79 Issue 3 Pages 858-863
A method for duodenal chymotrypsin determination was deviced using glutaryl-L-pheny-lalanine-p-nitroanilide(GPNA) as a substrate and diazo-reaction for colorimetry.
This GPNA method was simple, accurate and specific for duodenal chymotrypsin determination. Chymotrypsin activity obtained by the GPNA method correlated significantly with that by a benzoyl-L-tyrosine ethylester(BTEE) method.
Duodenal chymotrypsin was significantly inactivated at 37°C Cafter 15 minutes or at 20°C after 60 minutes, but it was stable at 0°C for 2 hours or at -20°C for 7 days.
Duodenal chymotrypsin output during pancreozymin secretin test had significant correlation with both amylase and trypsin outputs. In pancreatic cancer, chymotrypsin output decreased more than the amylase output. This dissociated reduction of chymotrypsin suggests that the chymotrypsin output can be a sensitive parameter for the exocrine pancreatic insufficiency caused by pancreatic cancer.
Nippon Shokakibyo Gakkai Zasshi
Nippon Shokakibyo Gakkai Zasshi Kou
