1982 Volume 79 Issue 5 Pages 1073-1083
In order to investigate the effects of vagotomy on gastrin (G) and somatostatin (D) cell population of a stomach and proliferation kinetics of G-cell, a quantitative analysis of G- and D- cell on truncal vagotomized rats was performed in comparison with that of control rats, by mean of immunoperoxidase identification technique combined with 3H-thymidine autoradiography. A radioimmunoassay was also performed to estimate the serum gastrin concentration both in the vagotomized rats and control rats. The results obtained as follows:
1) The basal serum gastrin values were significantly higher in vagotomized rats than control rats.
2) The total number of G-cells in a vagotomized stomach was more than in a control stomach, because the concentration of G-cell per unit mucosal surface did not become higher, but the extent of antral mucosal area became larger after the vagotomy.
3) The total number of D-cell both in corpus and antrum of a vagotomized stomach was significantly more than that of a control stomach.
4) It was suggested that a few percent of G-cells have a self-replicating activity and this activity was increased after the vagotomy, from the results that the laveling indices of 3Hthymidine flash lavel were 0.50-0.53% in control rats, 0.87-1.4% in the vagotomized rats.
5) It was thought that G-cell reproduction from immature gererative cells in the crypt was increased after a vagotomy from the results of 3H-thymidine cummurative autoradiographic studies.
Nippon Shokakibyo Gakkai Zasshi
Nippon Shokakibyo Gakkai Zasshi Kou
