ANTIGENICITY OF PHENOL WATER EXTRACT OF HUMAN FETAL COLON

Confirmation of antigenicity in polysaccharide fraction

Abstract

We have obtained the phenol water extract of the human colon according to the method of Broberger and Perlmann. This extract was reported by them to be antigenic as for circulating autoantibody in patients with ulcerative colitis. Moreovere we have already confirmed that this extract was an inducer of delayed reactivity in man and guinea pig. In this paper, antigenicity of this extract was examined.
This extract was treated with gel filtration technique using Sephadex G-200 and divided into polysaccharide (PS) fraction and OD280nm fraction.
Fifteen randombred guinea pigs were divided into three groups. The first group was immunized with PS fraction in Freunds Complete Adjuvant (FAC), the second group with OD280nm fraction in FCA, and the third group with saline in FCA.
After immunization, these guinea pigs were injected intradermally at their flanks with this phenol water extract, PS fraction, OD280nm fraction, E. coli 014 LPS, and saline, then intradermal reactions were examined timely.
In the first group, these reactions were positive with this extract and PS fraction, but were negative with OD280nm fraction. The same results were also found in the second group. In the third group, all these reactions were negative.
From this study, we confirmed that antigenicity of this extract was present in the polysaccharide fraction of this extract. Reactions against E. coli 014 LPS were positive in all these three groups.