新しい glutathione S-transferase の1分子種の精製

荻野 昌昭; 沖田 極; 名和田 浩; 安永 満; 新開 泰司; 門 裕二; 小田 正隆; 江崎 隆郎; 松田 彰史; 安藤 啓次郎; 野田 健一; 福本 陽平; 竹本 忠良

doi:10.11405/nisshoshi1964.81.1012

PURIFICATION OF A NEW GLUTATHIONE S-TRANSFERASE FROM RAT LIVER CYTOSOL AND ITS SIGNIFICANCE DURING HEPATOCARCINOGENESIS

Masaaki OGINO, Kiwamu OKITA, Hiroshi NAWATA, Mitsuru YASANAGA, Yasushi SHINGAI, Yuji KADO, Masataka ODA, Takarou EZAKI, Shoshi MATSUDA, Keijiro ANDO, Kenichi NODA, Youhei FUKUMOTO, Tadayoshi TAKEMOTO

Author information

Masaaki OGINO
First Division, Department of Internal Medicine, Yamaguchi University School of Medicine
Kiwamu OKITA
First Division, Department of Internal Medicine, Yamaguchi University School of Medicine
Hiroshi NAWATA
First Division, Department of Internal Medicine, Yamaguchi University School of Medicine
Mitsuru YASANAGA
First Division, Department of Internal Medicine, Yamaguchi University School of Medicine
Yasushi SHINGAI
First Division, Department of Internal Medicine, Yamaguchi University School of Medicine
Yuji KADO
First Division, Department of Internal Medicine, Yamaguchi University School of Medicine
Masataka ODA
First Division, Department of Internal Medicine, Yamaguchi University School of Medicine
Takarou EZAKI
First Division, Department of Internal Medicine, Yamaguchi University School of Medicine
Shoshi MATSUDA
First Division, Department of Internal Medicine, Yamaguchi University School of Medicine
Keijiro ANDO
First Division, Department of Internal Medicine, Yamaguchi University School of Medicine
Kenichi NODA
First Division, Department of Internal Medicine, Yamaguchi University School of Medicine
Youhei FUKUMOTO
First Division, Department of Internal Medicine, Yamaguchi University School of Medicine
Tadayoshi TAKEMOTO
First Division, Department of Internal Medicine, Yamaguchi University School of Medicine

Keywords: glutathione S-transferase

JOURNAL FREE ACCESS

1984 Volume 81 Issue 4 Pages 1012-1018

DOI https://doi.org/10.11405/nisshoshi1964.81.1012

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Abstract

A new form of glutathione S-transferase has been purified to homogenity from rat liver cytosol. Specific activity of this enzyme to the substrates such as 1-chloro-2, 4-dinitrobenzene, 1, 2-dichloro-4-nitrobenzene and bromosulfophthalein were different from that of transferase AA, A, B, C, E and ligandin previously reported. SDS-gel electrophoresis of this purified sample showed a single band corresponding to a molecular weight of 24, 300. From immunohistochemical sutdy, it was suggested that the enzyme may be induced in the hepatocytes during hepatocarcinogenesis.

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