ACTION OF NEUROMEDIN C ON AMYLASE RELEASE FROM ISOLATED RAT PANCREATIC ACINI

Abstract

We have studied the effect of neuromedin C, a novel decapeptide isolated from porcine spinal cord, on amylase secretion from isolated rat pancreatic acini. The effect of neuromedin C was detectable at concentration of 3pM, and the amylase release was increased in a dose-related manner. The maximal effect of neuromedin C was found at concentration of 1nM.
The concentration that produced half maximal effect (EC₅₀) was 45.3±4.5pM for neuromedin C. Atropine, muscarinic receptor antagonist, and proglumide, cholecystokinin receptor antagonist, did not affect on the response to neuromedin C.
These results suggest that neuromedin C acts directly on the rat pancreatic acinar cells, and the neuromedin C-induced pancreatic secretion is mediated via neither muscarinic nor cholecystokinin receptors.