Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
ENZYME IMMUNOASSAY FOR HUMAN EPIDERMAL GROWTH FACTOR
Takashi JOHMakoto ITOHKei MASTUSAKOKazuo IKEDASinpei IMAITadahisa MIYAMOTOYoshifumi YOKOYAMAKohei KASTUMIToshihiko TAKEUCHITaiji KATORyo TANAKA
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1987 Volume 84 Issue 1 Pages 84-91

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Abstract

We established a sandwich enzyme immunoassay (ETA) system for human epidermal growth factor, which has a high sensitivity (500fg/tube) and makes it possible to measure directly a minute amount of endogenous human EGF in biological fluids such as serum or urine. EGF in plasma was not detected (less than 0.05ng/ml). The concentrations of serum EGF in normal adult males and females were 0.57 and 0.46ng/ml respectively. Urinary EGF levels were 63.8ng/ml for males, and 87.2ng/ml for females. There was no correlation between serum and urinary EGF levels. It seems most likely that majority of urinary EGF is not derived from blood but from kidney itself.

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© The Japanese Society of Gastroenterology
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