A NEW GASTROINTESTINAL MUCINOUS ANTIGEN PREPARED BY USING A MONOCLONAL ANTIBODY, AND IT'S CHARACTERIZATION

Abstract

A new gastrointestinal antigen was obtained using the monoclonal antibody (MPB11) which was produced by hybridoma cells made by fusing mouse myeloma cells (P3U-1) with the spleen cells of a BALB/c mouse that had been immunized with suspended colonic cells in an F344 rat. Using the peroxidase labeled, antibody-staining method, the presence of the antigen against MPB11 was demonstrated in the surface mucin and crypt of the stomach, the small intestine, and the colon in the rat. It was not detected in the other organs i.e. esophagus, liver, kidneys, heart, lungs and spleen. In human tissues the antigen against MPB11 was demonstrated in the mucin in the stomach and duodenum, but colonic goblet cells did not have the antigen.
It's molecular weight was estimated at 60 kilodaltons by polyacrylamide gel electrophoresis and Western immunoblotting.
The presence of sialoprotein in the antigenic determinant was confirmed by examination with the enzymes (exoglycosidase, neuraminidase and pronase).