1987 Volume 84 Issue 9 Pages 1802-1812
A new pancreatic ductal cell antigen was identified by using a monoclonal antibody SP3-1 which was produced by hybridoma cells made by fusing mouse myeloma cells (P3U-1) with spleen cells of a BALB/c mouse immunized with pig pancreatic antigen. The homogenized pig pancreas was fractionated by gel filtration through Sephadex G-100 and the void fraction was absorbed with DEAE-Sephacel and then the 3rd eluted fraction was used for immunogen. Immunohistochemically, using the peroxidase labeled antibody-staining method, the antigen against SP3-1 was demonstrated in the pancreatic ductal cell, bile ductal cell, ductal cells of the salivary gland and the esophageal gland, and the renal tubules of the pig and human. The molecular weight of the recognized antigen was estimated at 60 kilo-daltons by polyacrylamide gel electrophoresis and Western immunoblotting. The varous enzyme treatments suggested that the antigenic determinant contained protein but not sialic acid or glucose, and that isotype of SP3-1 was IgM.