1989 Volume 86 Issue 7 Pages 1519-1524
The effects of oral and intraperitoneal administration of biotin in urease-induced hyperammonaemic rats, as well as the influence of biotin deficiency, have been studied.
Biotin deficiency was produced by feeding standard diet MF (Oriental Yeast Co.) supplemented with dry egg-white (egg-white group). Egg-white+biotin group had free access to 0.0014% of biotin solution at all time.
Following an intraperitoneal injection of urease, 25U/kg (B.W.), plasma ammonia levels in egg-white+biotin group were lower than in egg-white group, especially there was significance (p<0.05) at 8 hours after the urease injection. Similarly, plasma ammonia levels in biotin-injected rats, in which 1mg of biotin had been injected intraperitoneally prior to the experiment, were significantly low compared with saline-injected controls at 4 and 6 hours after urease administration.
Results of plasma amino acid analysis, 9 hours after the urease injection indicated that Fischer's molar ratio (Leu+Ileu+Val/Tyr+Phe) was significantly higher in the biotin-injected rats than the saline-injected control.
It suggests that biotin might decrease blood ammonia by facilitating the detoxification mechanism as follow: L-glutamate+NH3→L-glutamane.