1991 Volume 88 Issue 11 Pages 2749-2756
The biological significance of silver binding nucleolar organizer regions (Ag-NORs) had been evaluated in tissue sections from 21 colonic adenomas (11 mild atypia, 10 moderate atypia), 23 primary adenocarcinomas of the colon (12 intramucosal carcinomas, 11 advanced carcinomas) and 5 control cases, by automatic image analysis. A significant difference in the mean number of Ag-NORs per nucleus was found when adenomas with moderate atypia and carcinomas were compared with adenomas of mild atypia and control cases. The mean Ag-NORs area per 1 Ag-NOR of carcinomas tended to be larger than that of the adenomas and control group. The mean value of form factor in carcinomas showed greater pleomorphism in shape than that of adenomas and control group. The mean quotient of number and area of Ag-NOR (Ag-NOR score) of the carcinomas was highest among the cases examined.
These results indicate that evaluation of the mean Ag-NOR numbers is of great value, with regard to the cell proliferation, and that of Ag-NOR score may give the most accurate estimation of biological character in different groups of colonic neoplasia.