日本農芸化学会誌
Online ISSN : 1883-6844
Print ISSN : 0002-1407
ガスクロマトグラフィーによる単糖類のalditol-acetatesの分別定量における内部標準物質としてmethyl-β-D-glucosideの適合
日下部 功神山 由安井 恒男
著者情報
ジャーナル フリー

1977 年 51 巻 3 号 p. 167-169

詳細
抄録

This report deals with the application of methyl-β-D-glucoside as a more satisfactory internal standard in using gas-liquid chromatography (GLC) of monosaccharides-derived alditolacetates when performing quantitative analysis on monosaccharides.
Rhamnose and myoinositol is currently and widely used as an internal standard for quantitative GLC. However, the former is affected a great deal by tailing of solvents and acetylation reagents; while the latter requires an inconveniently long retention time. Moreover, both are normally found in the plant kingdom, for example in agricultural wastes and hence are not suitable for use as a standard.
Other sugars such as allose and idose although they are recommended by some authors, are not readily available in reasonable quantities.
Among all sugars and sugar derivatives tested in this study, methyl-β-D-glucoside was found to be the most satisfactory internal standard, since it appears midway on the chromatogram between D-xylose and D-mannose.
An advantage of using this glucoside is that it is stable and commercially available in the reagent grade quality. Moreover, chromatographic peak height ratios (monosaccharide/internal standard) is exactly proportional with regards to the weight ratios (monosaccharide/internal standard), and the glucoside is not normally found in the hydrolysates of the plant kingdom's natural raw materials.

著者関連情報
© 公益社団法人 日本農芸化学会
前の記事 次の記事
feedback
Top