1977 年 51 巻 3 号 p. 167-169
This report deals with the application of methyl-β-D-glucoside as a more satisfactory internal standard in using gas-liquid chromatography (GLC) of monosaccharides-derived alditolacetates when performing quantitative analysis on monosaccharides.
Rhamnose and myoinositol is currently and widely used as an internal standard for quantitative GLC. However, the former is affected a great deal by tailing of solvents and acetylation reagents; while the latter requires an inconveniently long retention time. Moreover, both are normally found in the plant kingdom, for example in agricultural wastes and hence are not suitable for use as a standard.
Other sugars such as allose and idose although they are recommended by some authors, are not readily available in reasonable quantities.
Among all sugars and sugar derivatives tested in this study, methyl-β-D-glucoside was found to be the most satisfactory internal standard, since it appears midway on the chromatogram between D-xylose and D-mannose.
An advantage of using this glucoside is that it is stable and commercially available in the reagent grade quality. Moreover, chromatographic peak height ratios (monosaccharide/internal standard) is exactly proportional with regards to the weight ratios (monosaccharide/internal standard), and the glucoside is not normally found in the hydrolysates of the plant kingdom's natural raw materials.