Abstract
Neutral lipids were isolated from Aspergillus oryzae, and chemical composition of the acyl lipid classes was analysed.
Neutral lipids consisted of hydrocarbon, sterol ester, fatty acid ester, triglyceride, free fatty acid, 4-methylsterol, 4-demethylsterol, 1, 3-diglyceride, 1, 2-diglyceride and three unidentified lipids; triglyceride and free fatty acid were predominant among them. The ratio of ethyl ester and methyl ester detected in fatty acid ester was approximately 58:42.
Main component fatty acids in neutral lipid, triglyceride and diglycerides were linoleic, oleic and palmitic acids in decreasing order. Sterol ester and fatty acid ester possessed a large quantity of linoleic acid. In free fatty acid a higher proportion of palmitic acid was observed than in other lipids.
As for the positional distribution of fatty acids in triglyceride, linoleic, oleic, palmitic and stearic acids were located at 1-C and 3-C of the molecule; unsaturated ones (oleic and linoleic acids) were predominant at 2-C.
Molecular species of triglyceride from monoenoic to hexaenoic type were found. Tetraenoic type (palmityldilinolein, palmityloleyllinolenin etc.) was the principal.
