Abstract
Carbohydrate metabolism during sporulation and germination of ascospores of Hasegawaea japonica was examined to detect changes in the synthesis and degradation of intracellular and wall materials. The cellular materials in spores during sporulation and germination were fractionated into spore-cytoplasm and spore walls. Trehalose in the spore-cytoplasm was synthesized during spore formation, and then the sugar was degraded during germination. Wall carbohydrate was fractionated into KOH-soluble glucan and -an insoluble fraction. The KOH-soluble glucan, as a major component of the spore walls, was synthesized during spore formation, and then the glucan was degraded during germination. The study found that trehalose and KOH-soluble glucan synthesized during sporulation of H. japonica were degraded during germination. The results were similar to the previous results for Saccharomyces cerevisiae.
