Nippon Nōgeikagaku Kaishi
Online ISSN : 1883-6844
Print ISSN : 0002-1407
ISSN-L : 0002-1407
A Simple Assay Method for α-Amylase Using Microplates
Toshiya SATOYAMAToshio HARAMasahiro MURATAYusaku FUJIO
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1998 Volume 72 Issue 8 Pages 933-936

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Abstract

A novel method for measuring α-amylase-activity was developed in this study. By adding an authentic α-amylase solution to a mixture of agar and gelatinized rice starch, which was fixed in a well of a microplate, the gel turbidity measured at 655 nm was decreased by the α-amylase action on the gelatinized starch. The intensity of the α-amylase-activity was in proportion logarithmically to the turbidity decrease detected by a microplate-reader. From reasonable values of standard deviation and coefficient of variation (3.7_??_8.9%) of the obtained data, this method makes it possible to measure α-amylase-activity conveniently.

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© JAPAN SOCIETY FOR BIOSCIENCE,BIOTECHNOLOGY, ANDAGROCHEMISTRY
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