日本食品科学工学会誌
Online ISSN : 1881-6681
Print ISSN : 1341-027X
ISSN-L : 1341-027X
シリーズ—地域食品研究のエクセレンス(第8回)
水溶性テトラゾリウム塩WSTを用いた微生物検出法の開発と食品分野への応用
塚谷 忠之
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ジャーナル フリー

2015 年 62 巻 7 号 p. 321-327

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To determine microbial populations and their viability, methods assessing colonies formed and turbidity are generally used. However, these methods require a long incubation time. Thus, a rapid colorimetric microbial viability assay based on the reduction of the tetrazolium salt WST-8 via 2-methyl-1,4-naphthoquinone as an electron mediator was developed. The applicability of the assay for susceptibility testing of various bacteria to antibiotics, screening of antimicrobial substances, determination of water-soluble vitamins, and differentiation of Gram-positive and -negative bacteria was investigated. Good agreement was observed between results with the WST-8 colorimetric method and those obtained using conventional methods. The present assay method was superior to the conventional methods with respect to the overall rapidity, accuracy and convenience.

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