Japan has become a “super-aged society” in which over 21% of the population is aged≥65 years. Many Japanese people are seeking functional foods that can prevent age-related diseases. Thus, we developed and applied a method of evaluating anti-aging foods based on age-related hearing loss (AHL) in mice. We found that the intake of a diet containing whole egg (8% or 20% of the diet) or 13.6% egg yolks for 6 months delayed the development of AHL, whereas a diet containing 6.4% egg whites had no effect. This indicates that egg yolk has the potential to prevent age-related diseases.