Antibacterial Activity of Tea Catechin to Streptococcus mutans
1989 Volume 36 Issue 6 Pages 463-467
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1989 Volume 36 Issue 6 Pages 463-467
Catechin fraction A (CF-A) containing (-)-epicatechin and (-)-epigallocatechin, fraction B (CF-B) containing (-)-epicatechin gallate and (-)-epigallocatechin gallate, CF-Aand CF-B mixture (CF-mix), and(-)-epigallocatechin gallate (EGCg) were prepared fromgreen tea by using the method of YAYABE et al., and the antibacterial activity of catechins onthe S. mutans was investigated. On the antibacterial effects to S. mutans, minimal inhibitoryconcentration (MIC) of CF-A, CF-B, CF-mix, and EGCg was 400-100, 100-50, 200-100, nd 100-50μg/ml, respectively. MIC of chlorohexidine gluconate solution was less than1.6ug/ml. On the sterilization effect, an addition of 20mg/ml of CF-B decreased the numberof S. mutans from 10<sup>7</sup>to 10<sup>2</sup> after 3min, while achlorohexidine gluconate solution showedabout 10 times higher sterilization activity. Although the antibacterial and sterilizationeffects of the CF-B were mild compared with those of chlorohexidine gluconate, it may beuseful as a natural antibacterial reagent for a dentifrice or a mouthwash liquid etc. due to itsreasonable antibacterial activity to S. mutans.
