2024 Volume 4 Issue 1 Pages 54-61
Purpose: Chemokine (C-C motif) ligand 28 (CCL28), the most recently identified chemokine, was reported in 2000 and has chemotactic and bactericidal activity. CCL28 is expressed in various tissues, including the salivary glands; however, whether CCL28 is expressed in human dental pulp is unclear. Therefore, this study investigated CCL28 expression in inflamed human dental pulp tissue and whether CCL28 and its receptor, C-C motif chemokine receptor 10 (CCR10), are expressed in cultured human dental pulp cells in response to inflammatory stimuli.
Methods: CCL28 expression in human dental pulp tissue was analyzed using immunohistochemistry. CCL28 and CCR10 mRNA and protein expression in cultured interleukin one beta (IL-1β) -stimulated human dental pulp cells was examined using real-time PCR and western blotting. CCL28 and CCR10 protein expression was analyzed using fluorescent immunocytochemical staining.
Results: In inflamed human dental pulp tissue, strong CCL28 signals were detected in dental pulp cells located at sites of intense inflammation accompanied by infiltration of inflammatory cells. Furthermore, IL-1β enhanced CCL28/CCR10 mRNA and protein expression in cultured human dental pulp cells. These findings suggest that CCL28 produced by dental pulp fibroblasts may act in an autocrine and paracrine manner during dental pulp inflammation and may be involved in the inflammatory cascade in the dental pulp.
Conclusion: CCL28/CCR10 may be involved in the immune response in inflamed human dental pulp. Understanding the role of CCL28 in dental pulp tissue provides insights into the progression of pulp inflammation through CCR10.
