抄録
The present study dealt with the influence of isolated hemoglobin upon the histochemical peroxidase reaction, and also with the application of this staining method to tissue sections. The materials used include cartilage, skin with hair, and mucosa of alimentary tract of 17 different vertebrates from mammal to fish, and benzidine and orthophenylenediamine were used for the substrates of peroxidase reaction. This new technique, called the indirect peroxidase reaction, is characterized by treating tissues with very dilute hemoglobin solution before the tissue is stained with peroxidase reagent.
It was revealed here that the interstitial substance of cartilage and the stratum germinativum of stratified squamous epithelium are intensely stained with the indirect peroxidase reaction, suggesting that the hemoglobin molecule is able to intensify the ordinary peroxidase reaction, and that the indirect peroxidase reaction is related to the basophilia of tissue, and perhaps as well to the proliferative activity of cells.
The prin c iple of the indirect peroxidase reaction is thought to be adsorption of hemoglobin molecules to a definite region of cell or tissue which causes a pseudoperoxidase reaction in various degrees.
Evidence was therefore presented that the animal p igment, hemoglobin, is applicable to tissue staining because of its high pseudoperoxidative activity.
