Okajimas Folia Anatomica Japonica
Online ISSN : 1881-1736
Print ISSN : 0030-154X
ISSN-L : 0030-154X
Histochemical Demonstration of Heavy Metals in the Hippocampal Formation Embedded in Quetol 523M
Yasukazu NAGATOMasaki SEKIGUCHIHiroshi KUSHIDAKazuyo SHIMAI
著者情報
ジャーナル フリー

1991 年 68 巻 5 号 p. 299-304

詳細
抄録

To facilitate improvement of investigations on the distribution of mossy fibers in the hippocampal formation, a method is described using Timm's stained preparations after methacrylate embedding with the hydrophilic resin, Quetol 523M. Fixation with a mixture of formaldehyde and glutaraldehyde yielded satisfactory staining results and good structural preservation. During the course of histochemical experiments employing Timm's staining, examinations revealed that sulfide silver reaction products were consistently present in both the mossy fibers themselves and their terminals associated with the dendrites of pyramidal cells in tissue sections of 1-2μm in thickness. The results obtained also revealed that variations of the mossy fiber system occurred in the neurological mutant mouse dreher (dr). The bundles of mossy fibers forming the intrapyramidal synaptic field may be considered to reflect genotype-dependent differences in the mutation. The present method is adequate for allowing the histochemical demonstration of mossy fibers and their giant boutons by light microscopy.

著者関連情報
© Editorial Board of Okajimas Folia Anatomica Japonica
前の記事
feedback
Top