Organ Biology
Online ISSN : 2188-0204
Print ISSN : 1340-5152
ISSN-L : 1340-5152
ナノバイオデバイスによるがん細胞由来エクソソーム解析
湯川 博馬場 嘉信
著者情報
ジャーナル フリー

2015 年 22 巻 2 号 p. 193-198

詳細
抄録

We herein showed the analysis of exosomes derived from cancer cells by nanobiodevices. At fi rst, the nano-pore device formed by an electrode couple on a nano-pore was developed. The number and size of exosomes could be analyzed by measuring the current value of the tunnel current of exosomes in the culture supernatant poured into nano-pore device. Next, the nano-wire device formed by nano-wires on a microfl uidic device made from polymethylmethacrylate (PMMA) was developed. The exosomes could be collected from the culture supernatant with high effi ciency by using this nano-wire device, and then miRNA existing in the exosomes could be extracted by pouring the miRNA extraction liquid into the nanowire device. In addition, these devices were available to the analysis of exosomes in the serum and urine. Therefore, the analysis of exosomes derived from cancer cells by nanobiodevices may be useful for the diagnosis of cancers in early stage.

著者関連情報
© 2015 日本臓器保存生物医学会
前の記事 次の記事
feedback
Top