Present status of large-scale culture methods of pluripotent stem cell : Differentiation of human iPS cells with dialysis suspension culture

Abstract

Pluripotent stem cells (PSCs) are expected to be a promising cell source in the field of regenerative medicine and cell-based drug screening because of their high self-renewal capability while preserving pluripotency. To realize these applications, large-scale and efficient cell production methodologies are highly required. In this short review, we introduced typical suspension culture methods with their advantages and disadvantages. In addition, we explained our latest trials concerning pancreatic β cells differentiation of human induced pluripotent stem cells (hiPSCs) using suspension culture system with dialysis operations.