Cell surface modulation of endothelial cells by cell surface engineering for improvement of graft survival in kidney transplantation

Abstract

Coagulation and complement systems in blood are activated during ischemia-reperfusion injury, resulting in cellular damage and thrombosis. This is one of the important factors which determine graft survival in transplantation of organ and cell. In this paper, I focused on the glycocalyx on endothelial cell surface, which is lost during ischemia-reperfusion injury, and aimed to make the coating sugar materials alternative to the glycocalyx for prevention of coagulation and complement activation. I used polyethylene glycol-conjugated lipid (PEG-lipid) for the cell surface modification and succeeded in the heparinization of the cellular surface. Here I introduced two methods for the heparinization. By using the heparinization methods, it was possible to suppress the coagulation activation in human blood triggered by hMSC, indicating that our heparinization could contribute to the cell protection during ischemia-reperfusion injury in transplantation of organ and cell.