Abstract
Human nasal secretion was studied by means of immunoelectrophoresis in order to compare the same to the serum and to investigate distribution of protein fraction in various nasoparanasal diseases.
The two different antiserums, antihuman serum rabbit serum and anti-nasal secretion rabbit serum, were prepared to analyze the protein in the nasal secretion.
Each fraction of protein was identified according to Quchtherlony's method, thereby comparisons were made among the nasal secretions obtained in various diseases of the nasoparanasal diseases.
The nasal secretion obtained carefully excluding blood component was centrifuged to determine the protein concentration and then studied by means of immunoelectrophoresis.
The results of the immunoelectrophoresis of nasal secretion may be summarized as follows;
1. Nasal secretion contained albumin, haptoglobin, transferrin, IgA and IgG.
2. Watery nasal secretion and mucoid nasal secretion were often found to lack haptoglobin and transferrin, while mucopurulent or purulent discharge also lacked haptoglobin and transferrin but less frequently. Serous secretion always contained all of the five proteins.
3. Haptoglobin and transferrin were often missing in so-called unstable stage of chronic sinusitis.
4. All the five proteins, albumin, haptoglobin, transferrin, IgA, and IgG were identified in almost all postoperative cases of chronic sinusitis.
5. The above five proteins were likewise proved in reactions between nasal secretion and antinasal secretion rabbit serum.
6. No data were available as to indicate the presence of specific protein in the nasal secretion as studied by reactions between human serum and anti-nasal secretion rabbit serum or nasal secretion and anti-nasal secretion rabbit seum.
