実験的蝸牛窓膜破裂症の組織学的研究
1989 年 32 巻 2 号 p. 141-150
詳細
1989 年 32 巻 2 号 p. 141-150
We made experimental animal model of perilymphatic fistula and observed it histopathologically.
Perilymphatic fistula was produced by the injection of artificial perilymph into the subarachnoid space of posterior fossa. Rupture of the round window was confirmed by direct observation of the round window membrane under a surgical microscope during the injection. The animals were either vitally fixed immediately after injection or kept alive for 1 to 3 months before fixation. For histopathological study, the conventional celloidin embedding method was used for serial sectioning. Various types and degrees of changes in the membrana labyrinth were observed. In immediate observation, ten cochleas showed hydrops, seven showed collapse, and 21 of 38 cochleas showed no change. Changes in the vestibular apparatus varied, with collapse of the membranous vestibular labyrihth as the main change. Delayed observation revealed 17 normal cochleas and four collapses and one marked hydrops out of 22 ears. Of these 22, ten ears showed normal vestibular apparatus, ten collapse, and two saccule hydrops. The marked cochlear hydrops was produced by blöcking the longitudinal flow of endolymph. Loss of the outer hair cells was observed in six of 22 cochleas. Marked compression of the örgan of Corti was seen in both immediate and delayed obsevations.
