Ultrastructural Localization of sialic acids on the guinea pig eustachian tube and middle ear mucosa was studied using lectin-gold technique of Limax Flavus Aggutinine. Under normal conditions, sialic acids were detected in the secretory granules of the goblet cells, on the plasma membrane of the cilia and microvilli of the ciliated cells. The periciliary space was margined with membranous structure and sialic acids were distributed on its inner surface. On the other hand, in the case of experimental otitis media with effusion, the mucous layer covered the surface of the cilias in which a large amount of sialic acids was detected.
