1997 年 7 巻 3 号 p. 144-148
Effects of epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) on healing proces of the tympanic membrane perforation were investigated in guinea pigs. The tympanic membrane perforations were examined histologically and immunohistochemically with bromodeoxyuridine and labeling index of bromodeoxyuridine at the margin of perforation was calculated.
The tympanic membrane perforations were closed more rapidly in the PDGF-treated than in the EGF-treated ears. Significant proliferation of the epithelial layer was obtained with both treatments. However, proliferation of the middle layer of the tympanic membrane was greater in the PDGFtreated than the EGF-treated ears. These results suggested that proliferation of the middle layer stimulates the epithelial layer and that proliferation of the epithelial layer directly fastens the closure of perforation of the tympanic membrane.