PAIN RESEARCH
Online ISSN : 2187-4697
Print ISSN : 0915-8588
ISSN-L : 0915-8588
原著
Evaluation of restriction of movement and hyperalgesia in an only–ankle–immobilization rat model
Takeshi KijimaTakako Nakanishi–UedaNorio YoshidaYuuri YoshidaNoriyuki SeradaMasataka SunagawaTadashi Hisamitsu
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ジャーナル フリー

2014 年 29 巻 1 号 p. 51-56

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   The purpose of this study was to establish an only–ankle–immobilization model. We divided 10 Wistar male rats into a control group (Cont, n=5) and immobilization group (IM, n=5). IM had their right ankle immobilized in planter flexion (PF) using external fixation for 4 weeks, while Cont were observed without immobilization for 4 weeks. All rats gained weight during the 4 weeks without significant differences between groups. Range of motion (ROM), pain threshold, and body weight were measured in all rats once a week for the 4 weeks. ROM of ankle dorsiflexion (DF) and PF, knee extension, and flexion were measured using X–ray fluoroscopy with a tension gauge, and pain thresholds were evaluated by behavioral response with the von Frey test and Hargreaves Assay using a plantar test. All data were shown as % of right limb ⁄ left limb. Ankle DF in IM was significantly limited 1 week after immobilization (83.7% vs. Cont: 98%, p<0.05), and it continued decrease to 70.4%, 51.5%, and 61.9% after 2, 3, and 4 weeks, respective­ly (vs. Cont: 99.4%, p<0.01). Ankle PF, knee extension, and flexion showed no significant difference between the groups. In the von Frey test, the mechanical hyperalgesia threshold in IM was significantly decreased to 45.8% after 1 week compared to the Cont rats (90.4%, p<0.01), and then further to 21.4%, 17.7%, and 20% after 2, 3, and 4 weeks, respectively (vs. Cont, p<0.01). In the plantar test, thermal nociceptive thresholds significantly decreased to 62.9% after 1 week in IM (vs. Cont: 96.7%, p<0.01), and then further to 83.3%, 70.6%, and 76% after 2, 3, and 4 weeks, respectively (vs. Cont, p<0.01). The model shown here will be useful for further studies to investigate the mechanisms and treatments of immobilization.

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© 2014 Japanese Association for the Study of Pain
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