The outcomes of endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) for suspected postoperative recurrence of biliopancreatic malignant tumors from 2018 to 2021 were investigated, including eight cases. The median age was 68 years (range: 45-85), including five males and three females (five with pancreatic cancer, one with pancreatic neuroendocrine tumor, one with distal bile duct cancer, and one with intraductal papillary mucinous carcinoma). Target lesions included five remnant pancreas, one soft tissue around the celiac artery, one liver, and one mediastinal lymph node. The median lesion diameter was 15 mm, and the needle gauge was 22 G in six cases and 25 G in two cases. Pathological diagnosis was possible in all cases, with no adverse events. EUS-FNA is a powerful modality to diagnose the recurrence of biliopancreatic malignancies.