Preparation of PEG and Protein Co-immobilized Upconversion Nanophosphors as Near-infrared Biolabeling Materials

Masao Kamimura; Daisuke Miyamoto; Yu Saito; Kohei Soga; Yukio Nagasaki

doi:10.2494/photopolymer.21.183

Masao Kamimura, Daisuke Miyamoto, Yu Saito, Kohei Soga, Yukio Nagasaki

著者情報

Masao Kamimura
Graduate School of Pure and Applied Sciences, University of Tsukuba
Daisuke Miyamoto
Graduate School of Pure and Applied Sciences, University of Tsukuba
sukuba Research Center for Interdisciplinary Materials Sciences (TIMS), University of Tsukuba
Center for Tsukuba Advanced Research Alliance (TARA), University of Tsukuba
Yu Saito
Department of Materials Science and Technology, Tokyo University of Science
Kohei Soga
Center for Tsukuba Advanced Research Alliance (TARA), University of Tsukuba
Department of Materials Science and Technology, Tokyo University of Science
Polyscale Technology Research Center (PTRC), Tokyo University of Science
Yukio Nagasaki
Graduate School of Pure and Applied Sciences, University of Tsukuba
sukuba Research Center for Interdisciplinary Materials Sciences (TIMS), University of Tsukuba
Center for Tsukuba Advanced Research Alliance (TARA), University of Tsukuba
Polyscale Technology Research Center (PTRC), Tokyo University of Science
Master's School of Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba
Satellite Laboratory, International Center for Materials Nanoarchitectonics (MANA), National Institute of Materials Science (NIMS)

キーワード: upconversion, bio-imaging, inorganic-polymer composite, nanoparticles, PEG

ジャーナルフリー

2008 年 21 巻 2 号 p. 183-187

DOI https://doi.org/10.2494/photopolymer.21.183

詳細

抄録

Upconversion phosphors are expected to be used as fluorescence biolabeling materials. One of upconversion phosphors, erbium ion-doped-Y₂O₃ (Y₂O₃:Er) nanoparticles (YNPs) was synthesized by homogeneous precipitation method. Poly(ethylene glycol)-b-Poly(acrylic acid) (PEG-b-PAAc) was immobilized on the surface of YNP to improve dispersing stability and prevention of nonspecific adsorption. In order to install protein on the YNP surface, co-immobilization of PEG-b-PAAc and bovine serum albumin (BSA) as a model protein was carried out. The specific molecular recognition of PEG/BSA co-immobilized YNPs (PEG/BSA-YNPs) was confirmed by anti-BSA antibody plate assay. The obtained complex is a promising near-infrared biolabeling materials with high performances.

責任著者(Corresponding author)

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