2016 Volume 29 Issue 3 Pages 413-418
To examine the effects of hydrogenated-tetrahedral amorphous carbon (ta-C:H) on differentiation of osteoblasts and osteoclasts, osteoblastic MC3T3-E1 cells and osteoclastic RAW264.7 cells were cultured on these titanium (Ti) disks with various surface roughness. Real-time quantitative reverse transcriptase-polymerase chain reaction analysis showed that the mRNA expressions of Runx2 and ALP, used as osteoblast differentiation markers, were significantly higher in MC3T3-E1 cells cultured on ta-C:H-coated Ti disks (ta-C:H Ti). On the other side, the mRNA expressions of TRAP and cathepsin K, which are osteoclast differentiation markers, were significantly lower in RANKL-treated RAW264.7 cells. These results, taken together, suggested the possibility that ta-C:H Ti simultaneously altered osteoblast and osteoclast differentiation, which could be preferable for osseointegration acquisition and long-term success of implant therapy.
