2003 Volume 79B Issue 1 Pages 15-21
The complete genomic DNA sequence of the thermophilic archaeon Thermoplasma volcanium is visually represented by assembling micro-squares in four color-types. The number of bases in this sequence (1,584,804) is larger than what was reported earlier (1,584,799 in Kawashima, T., Yamamoto, Y., Aramaki, H., Nunoshiba, T., Kawamoto, T. et al., 1999, Proc. Japan Acad. 75B, 213-218) by 5. These five bases (A at position 320,375, C at 342,507, T at 1,182,809, A at 1,365,060, and C at 1,376,292 in the new version) are missing from the previous version, mainly, not because of erroneous sequence reading, but due to physical deletion of corresponding base-pairs upon replication of the cloned DNA fragments in E. coli cells.
(Communicated by Masanori OTSUKA, M. J. A., Jan. 14, 2003)