1993 Volume 69 Issue 3 Pages 65-69
Fatty acids were released from the total lipids of Helicobacter pylori by acid methanolysis and analyzed by gas liquid chromatography and mass spectrometry. Eight peaks of the fatty acid methyl esters were detected and identified to be tetradecanoic, hexadecanoic, octadecanoic, octadecenoic, octadecadienoic, cyclo [11, 12] methylene octadecanoic, 11-methoxy-heptadecanoic and 11-methoxy-nonadecanoic fatty acids. A small amount of hydroxy docosanoic acid was also detected after trimethylsilylation. Two fatty acids, 11-methoxy-heptadecanoic and 11-methoxy-nonadecanoic acids, are unique and the occurrence of these fatty acids in bacteria as well as Helicobacter pylori is reported for the first time.
