Proceedings of the Japan Academy, Series B
Online ISSN : 1349-2896
Print ISSN : 0386-2208
ISSN-L : 0386-2208
Basic construction of the functional system in a protein
I. Functional system of modified trypsin (m-trypsin)
Yukio KIHOChristopher PALMERMasami ITOTsuruji IWAIYoshio OKADA
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1999 Volume 75 Issue 6 Pages 144-149

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Abstract

Modified trypsin (m-trysin) (essential amino acids for human in trypsin are neglected by replacing them with G (dummy)) was analysed by DEV model. It was found that the proteolytic functional system of trypsin could be assigned with m-trypsin and essential amino acids are not necessary in this step. Similar results were obtained with m-RNase and non-polar trypsin. These results suggest that the basic plan in the construction of trypsin or RNase can be made from non-polar or non-essential amino acids. The possible involvement of water is also discussed. Further discussion on the basic plan clarified the necessity for large DEV and DD values. Large DEV values for site B can be attained mainly by large n(z) values in formula [1] and are neutralized by large DD values from site E.

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