Proceedings of the Japan Academy, Series B
Online ISSN : 1349-2896
Print ISSN : 0386-2208
ISSN-L : 0386-2208
Hyperploidy of embryonic fibroblasts derived from Parp-1 knockout mouse
Tadashige NOZAKIHisako FUJIHARANobuo KAMADAOtoya UEDATsuyoshi TAKATOHitoshi NAKAGAMATakashi SUGIMURAHiroshi SUZUKIMitsuko MASUTANI
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2001 Volume 77 Issue 6 Pages 121-124

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Abstract

Various studies show that poly(ADP-ribose) polymerase-1 (Parp-1) is involved in genomic stability. We previously established Parp-1 knockout (Parp-1-1-) mice by disrupting Parp-1 exon 1. Parp-1-1- and Parp-1+/+ embryonic fibroblasts were isolated and they became immortalized spontaneously after two months of culture. Parp-1-1- embryonic fibroblasts showed extensive hyperploidy compared to Parp-1+1+ counterparts, and most of the cells contained a single nucleus. These results suggest that disruption of Parp-1 could result in the perturbation of chromosomal ploidy control through endoreduplication.

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